Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 31-61 of Human GALNT5.
Mouse spleen tissue lysate
IHC-human normal lung tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103324 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 106 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates.
Cell Membrane and Golgi Apparatus
GALNAC T5 antibody
N acetylgalactosaminyltransferase antibody
Polypeptide GalNAc transferase 5 antibody
Western blot - Anti-GALNT5 antibody (ab103324)
Anti-GALNT5 antibody (ab103324) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg
Predicted band size: 106 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT5 antibody (ab103324)
ab103324, at 1/50 dilution, staining GALNT5 in formalin-fixed, paraffin-embedded human lung tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"