Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Wholemount brain tissue)
Specification
Wholemount brain tissue
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Diluent: PBS with 5mM sodium azide and 1% TX-100
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 633 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Obtained nuclear labeling in all cell bodies (magenta in associated figure) for a fly line in which Gal4 expression is restricted to a small number of cells (green in the associated figure). Flies were maintained at 24oC which produces robust Gal4-driven GFP expression (see associated figure).
Abcam response
Thank you very much for the feedback provided and for participating to our AbTrial testing program. We are sorry to hear that this antibody did not provide you with satisfactory results in IHC-Wholemount using fruit fly samples. Since this antibody has been previously tested in Drosophila by other customers with negative results, we do think that this antibody is not suitable for this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 06 2014