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How would I account for endogenous long-chain Acyl-CoAs that are already in my tissue extract samples?
Asked on Jun 24 2014
You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogenous Acyl-CoAs, you can subtract this background from the +ACS Reagent wells.
Caitlin Abcam Scientific Support
回复于 Jun 24 2014