Question (75630) | Free Fatty Acid Assay Kit - Quantification (ab65341)

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Question

Does tissue need to be fresh or can we snap freeze in liquid nitrogen and grind a small amount prior to homogenising in chloroform/Tx100?


Answer


Although we would suggest to use fresh tissue preferably, tissue that is snap-frozen in liquid nitrogen can be used too. The tissue can be ground and then lipid extraction can be done with choloroform-triton.

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