产品名称Free Fatty Acid Assay试剂盒- Quantification
参阅全部 Fatty acid 试剂盒
样品类型Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
灵敏度> 2 µM
Free Fatty Acid Quantification Assay Kit ab65341 provides a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids (FA) in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.
In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
Visit our FAQs page for tips and troubleshooting.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
This assay detects formation of C-8 (octanoate) and longer fatty acids.
存放说明Store at -20°C. Please refer to protocols.
组件 标识符 100 tests ACS Reagent Blue 1 vial Enhancer Purple 1 x 200µl Fatty Acid Assay Buffer WM 1 x 25ml Fatty Acid enzyme mix Green 1 vial Fatty Acid Probe (in DMSO) Red 1 x 200µl Palmitic Acid Standard (1nmol/µl) Yellow 1 x 300µl
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
相关性Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response.
Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
Free Fatty Acid measured in biologicals showing concentration (µM)
Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
This product has been referenced in:
- Harm S et al. Removal of stabilizers from human serum albumin by adsorbents and dialysis used in blood purification. PLoS One 13:e0191741 (2018). Human . Read more (PubMed: 29364955) »
- Shimazu T et al. Role of METTL20 in regulating ß-oxidation and heat production in mice under fasting or ketogenic conditions. Sci Rep 8:1179 (2018). Read more (PubMed: 29352221) »