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Our Abpromise guarantee covers the use of ab11959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/500 - 1/5000.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).|
ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"