antigen: MDSQKEALQR IISTLANKND EIQNFIDTLH HTLKGVQENS SNILSELDEE FDSLYSILDE VKESMINCIK QEQA, corresponding to amino acids 1-74 of Human FSD1L Isoform 2 (Q9BXM9-2).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain. Contains 1 fibronectin type-III domain.
Information by UniProt
Coiled coil domain containing 10 antibody
Coiled-coil domain-containing protein 10 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FSD1L antibody (ab122254)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122254 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSD1L antibody (ab122254)
ab122254, at 1/400, staining FSD1L in paraffin embedded Human duodenum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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