重组Anti-Extracellular matrix蛋白1抗体[EPR6701] (ab126629)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Extracellular matrix蛋白1抗体[EPR6701]
参阅全部 Extracellular matrix protein 1 一抗 -
描述
兔单克隆抗体[EPR6701] to Extracellular matrix蛋白1 -
宿主
Rabbit -
特异性
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.
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经测试应用
适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. The exact sequence is proprietary.
Database link: Q16610 -
阳性对照
- WB: MDA MB 435 cell line, Rat heart, Rat kidney, Human skin cell, and A375 cell line lysates. IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt (intra): A375 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.20 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6701 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126629于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/20.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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IP |
1/10 - 1/100.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
ICC/IF | (1) |
1/100 - 1/250.
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说明 |
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Flow Cyt (Intra)
1/20. |
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. -
组织特异性
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. -
疾病相关
Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1893 Human
- Entrez Gene: 116662 Rat
- Omim: 602201 Human
- SwissProt: Q16610 Human
- SwissProt: Q62894 Rat
- Unigene: 81071 Human
- Unigene: 97792 Rat
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别名
- ECM 1 antibody
- Ecm1 antibody
- ECM1_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution (Purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell)
Lane 2 : Rat heart lysates
Lane 3 : Rat kidney lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa -
ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126629 & A375 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution
Lane 1 : MDA MB 435 cell line lysate
Lane 2 : Human skin cell lysate
Lane 3 : A375 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution
Predicted band size: 61 kDa -
ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab126629 被引用在 9 文献中.
- Guo Y et al. Eliminating the original cargos of glioblastoma cell-derived small extracellular vesicles for efficient drug delivery to glioblastoma with improved biosafety. Bioact Mater 16:204-217 (2022). PubMed: 35386327
- Lv C et al. Wentilactone A Reverses the NF-κB/ECM1 Signaling-Induced Cisplatin Resistance through Inhibition of IKK/IκB in Ovarian Cancer Cells. Nutrients 14:N/A (2022). PubMed: 36145166
- Dai Z et al. Brusatol Inhibits Proliferation and Invasion of Glioblastoma by Down-Regulating the Expression of ECM1. Front Pharmacol 12:775680 (2021). PubMed: 34970146
- Zhang B et al. Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization via the ECM1/STAT5 Pathway. Front Med (Lausanne) 8:796743 (2021). PubMed: 35141243
- Yang H et al. MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1. Biosci Rep 40:N/A (2020). PubMed: 32401301
- Liu X et al. Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction. Andrology 8:407-420 (2020). PubMed: 31364287
- Li W et al. Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NF?B/c-Myc signaling in female esophageal carcinoma. Mol Cancer 18:22 (2019). PubMed: 30736860
- Fitzgerald HC et al. Idiopathic infertility in women is associated with distinct changes in proliferative phase uterine fluid proteins. Biol Reprod 98:752-764 (2018). PubMed: 29546322
- Ziegler YS et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . PubMed: 27355404