概述

性能

应用

Our Abpromise guarantee covers the use of ab19331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 18179891
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.

靶标

  • 功能
    Nuclear receptor, may regulate ESR1 transcriptional activity.
  • 疾病相关
    Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Err 2 antibody
    • ERR b antibody
    • ERR B2 antibody
    • ERR beta 2 antibody
    • ERR beta antibody
    • ERR beta-2 antibody
    • ERR-beta antibody
    • Err2 antibody
    • ERR2_HUMAN antibody
    • ERRB 2 antibody
    • ERRb antibody
    • ERRB2 antibody
    • ERRbeta 2 antibody
    • ERRbeta antibody
    • ESR L2 antibody
    • ESRL 2 antibody
    • ESRL2 antibody
    • Esrrb antibody
    • Estrogen receptor like 2 antibody
    • Estrogen receptor related 2 antibody
    • Estrogen receptor-like 2 antibody
    • Estrogen-related receptor beta antibody
    • Estrrb antibody
    • Nr3b2 antibody
    • Nuclear receptor ERRB2 antibody
    • Nuclear receptor subfamily 3 group B member 2 antibody
    • Orphan nuclear receptor antibody
    • Steroid hormone receptor ERR 2 antibody
    • Steroid hormone receptor ERR2 antibody
    see all

图片

  • Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).

  • ab19331 staining Estrogen Related Receptor Beta protein on mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/200, was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Pacwa A  et al. Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study. Acta Histochem 120:456-467 (2018). WB ; Rat . Read more (PubMed: 29778238) »
  • Kotula-Balak M  et al. Insights into the role of estrogen-related receptors a, ß and ? in tumor Leydig cells. Tissue Cell 52:78-91 (2018). Read more (PubMed: 29857832) »
See all 6 Publications for this product

客户评价及客户问答

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 22 2015

Answer

Thank you for your enquiry regarding ab19331.

I can confirm that this antibody has been characterized for IHC-Fr (on frozen tissue), ICC/IF (on cultured cells or cytospin preparations) and in recognizes Estrogen Related Receptor beta in mouse. This receptor is expressed in ovary and the customer may wish to check the following website for further information on the expression profile:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.235550

I would advise your customer to do some literature search (PubMed) to find out what sort of studies have been carried out specifically on murine ovary to monitor developmental expression of Estrogen Related Receptor beta.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Fixative
methanol:acetone 1:1
Permeabilization
No
Blocking step
0.1%BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Nov 24 2011

Answer

Thank you for your enquiry regarding our Estrogen Related Receptor beta antibody (ab19331) I can confirm the western blotting procedure used for testing this antibody was as follows: 1) Block membrane with 5 ml of blocking buffer for 1 hour at room temp., gently agitating solution. 2) Incubate primary antibody diluted 1:250 in blocking buffer for 1 hour at room temp. gently agitating solution. Peptide Pre-incubations: Incubate primary antibody in 100 ul of blocking buffer for 15 min. at room temp with peptide prior to incubation with filter. 3) Wash filter 3X with TBST buffer, 8 min. at room temp. each, gently agitating solution. 4) Incubate secondary antibody diluted 1:30,000 in blocking buffer for 1 hour at room temp. gently agitating solution. 5) Wash filter 3X with TBST buffer, 8 min. at room temp. each, gently agitating solution. 6) Visualize by ECL detection and expose film. Good positive controls for this antibody include: Rat kidney, testes and skeletal muscle. Unfortuantely, I am sorry we do not have a blocking peptide available for this particular antibody. I hope this information is helpful. Should you have any further questions, please do not hestiate to contact us again.

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Answer

I'm very sorry to hear you are experiencing problems with ab19331, ab12986 and ab12987. I have looked at the shipping times of your order and would like to reassure you that there were no delays in the shipping. It is not uncommon that antibodies arrive at room temperature, especially when they travel some long distances and we have done stability studies in our laboratories and found that activity will not be impaired by such conditions. A high background problem is often due to the blocking agent or the secondary antibody; in our laboratory we block with 5% BSA (1hr) and find this buffer gives us the best results, while milk can give background problems so I think switching to BSA will help you a lot. Have you also tested if the secondary antibody works well with other primaries and if alone(no primary)it gives you some staining? For ab19331 the antibody has been tested in rat kidney samples and therefore I would like to recommend running this positive control as well as doing a nuclear extraction from your samples to concentrate the protein as you may have low levels of protein (you do not mention what cells you are studying), as well as incubating the antibody overnight in TBST. The antibodies ab12986 and ab12987 have not been tested in WB and therefore it is very difficult to know if those antibodies should work in this application and I would recommend trying the same protocol advice given above for ab19331. These antibodies have only been tested in human tissue so a human positive control is essential (e.g testis tissue). I think the above suggestions should help you a lot, if you still experience problems please do not hesitate to contact us. Ab19331 is guaranteed to work in WB in rat samples and if you still experience problems with this antibody in this application and species with the above changes I can offer you a replacement or refund,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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