Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Uterus)
Specification
Uterus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer (pH 6) at 90C for 20 min
Permeabilization
Yes - TBST Triton 100X
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Concentration
10 µg/ml
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 1% BSA
Secondary antibody
Name
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Roza Yunusova
Verified customer
提交于 May 19 2011