Application
ChIP
Sample
Human Cell lysate - nuclear (mammary cell line (MCF7))
Specification
mammary cell line (MCF7)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
Estrogen treated MCF7 cells tested at PS2 promoter
Negative control
IgG ChIP and ethanol-depleted cells tested at PS2 promoter
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 14 2013