重组PE Anti-Estrogen Receptor alpha抗体[E115] (ab209288)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [E115] to Estrogen Receptor alpha
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Estrogen Receptor alpha抗体[E115]
参阅全部 Estrogen Receptor alpha 一抗 -
描述
PE兔单克隆抗体[E115] to Estrogen Receptor alpha -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
Expression levels of ER alpha protein vary with sample type. -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (Intra): T47D, 4T1 and GH3 cells. ICC/IF: T47D cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E115 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab209288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50 - 1/2500.
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ICC/IF |
1/500.
This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) |
说明 |
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Flow Cyt (Intra)
1/50 - 1/2500. |
ICC/IF
1/500. This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) |
靶标
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功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- SwissProt: P06211 Rat
- Unigene: 208124 Human
see all -
别名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
图片
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ab209288 staining Estrogen Receptor alpha in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209288 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Overlay histogram showing T47D cells stained with ab209288 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209288, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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Intracellular Flow Cytometry analysis of 4T1 (mouse mammary gland carcinoma epithelial cell) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Intracellular Flow Cytometry analysis of GH3 (rat pituitary epithelial) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab209288 被引用在 2 文献中.
- Zhao Y et al. Circulating Innate Lymphoid Cells Exhibit Distinctive Distribution During Normal Pregnancy. Reprod Sci 29:1124-1135 (2022). PubMed: 34988918
- Kim J et al. Chronic and Low Dose Exposure to Nonlyphenol or Di(2-Ethylhexyl) Phthalate Alters Cell Proliferation and the Localization of Steroid Hormone Receptors in Uterine Endometria in Mice. Dev Reprod 23:263-275 (2019). PubMed: 31660453