Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 antibody [EPR17526]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were left untreated, or treated with either PBDu (1uM 30min) or U0126 (10uM 1 h), paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Following PDBu treatment, this antibody appears to become highly enriched within nuclei versus a more diffuse and equally distributed pattern (cytoplasm + nucleus) in untreated cells. Following U0126 treatment, this antibody does not appear to change localization and appears similar to the untreated condition.

Dr. Kirk Mcmanus

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提交于 Jul 26 2017

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