概述

  • 产品名称
  • 描述
    兔多克隆抗体to EHHADH
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of Human EHHADH (NP_001957.2).

  • 阳性对照
    • WB: mouse liver and kidney tissue lysates IHC-P: human hepatocarcinoma

性能

应用

Our Abpromise guarantee covers the use of ab93172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 79 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 组织特异性
    Liver and kidney. Lower amounts seen in the brain.
  • 通路
    Lipid metabolism; fatty acid beta-oxidation.
  • 序列相似性
    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 翻译后修饰
    Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • 细胞定位
    Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

图片

  • All lanes : Anti-EHHADH antibody (ab93172) at 1/100 dilution

    Lane 1 : mouse liver tissue lysates
    Lane 2 : mouse kidney tissue lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 79 kDa

  • ab93172, at a 1/50 dilution, staining EHHADH in formalin-fixed and paraffin-embedded Human hepatocarcinoma peroxidase-conjugated to a secondary antibody, followed by DAB staining.

文献

This product has been referenced in:
  • Tsoli M  et al. Depletion of white adipose tissue in cancer cachexia syndrome is associated with inflammatory signaling and disrupted circadian regulation. PLoS One 9:e92966 (2014). WB ; Mouse . Read more (PubMed: 24667661) »
See 1 Publication for this product

客户评价及客户问答

Answer

Thank you for your inquiry and I am sorry to hear that you are having some trouble with this antibody.

We sell the mouse liver whole cell lysate as ab29301:

https://www.abcam.com/Liver-Mouse-Whole-Cell-Lysate-normal-tissue-ab29301.html

I would recommend purchasing the positive control. If you're still not seeing bands with the antibody using the positive control, let me know and we can replace or refund the antibody if it was purchased within the last 6 months.

I hope this information helps. Please contact us with any other questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册