Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1 µg/ml
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 37, 125 kDa
Positive control
rat brain
Additional data
Additional Notes
We speculate these bands as right bands but further confirmation from abcam experts is required.
Abcam response
Thank you for submitting your review on this untested species. The band does look to be the correct MW. You may wish to use the blocking peptide ab127026 to demonstrate specificity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 23 2015