概述

  • 产品名称
    Anti-Dopamine D2 Receptor抗体
    参阅全部 Dopamine D2 Receptor 一抗
  • 描述
    兔多克隆抗体to Dopamine D2 Receptor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Dopamine D2 Receptor.

    参阅Abcam的专有抗源政策 (Peptide available as ab97357.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.

性能

应用

Our Abpromise guarantee covers the use of ab85367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能
    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 疾病相关
    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

图片

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Rat Substantia Nigra Lysate
    Lane 2 : Dopaminergic nucleus Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?
    Additional bands at: 52 kDa (possible glycosylated form)


    Exposure time: 4 minutes

实验方案

文献

This product has been referenced in:
  • Shen F  et al. Distinctive Changes of L-Type Calcium Channels and Dopamine Receptors in the Dorsomedial and Dorsolateral Striatum after the Expression of Habitual Cocaine-Seeking Behavior in Rats. Neuroscience 370:139-147 (2018). WB ; Rat . Read more (PubMed: 28764937) »
  • Chun S  et al. Thalamic miR-338-3p mediates auditory thalamocortical disruption and its late onset in models of 22q11.2 microdeletion. Nat Med 23:39-48 (2017). Read more (PubMed: 27892953) »
See all 4 Publications for this product

客户评价及客户问答

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (AML3)
Permeabilization
Yes - 0.5% Triton
Gating Strategy
Live cells
Specification
AML3
Fixation
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y)
Permeabilization
Yes - TRITON X-100 (0'25%)
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Feb 02 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

提交于 Feb 08 2012

Answer

Thank you for contacting us.  Unfortunately, I was unable to locate any amino acid sequences for Tilapia S100, S100 beta, or Dopamine D2 Receptor with which to compare the immunogen sequences for these antibodies.  For this reason, we have no information about whether or not these antibodies might react with Tilapia.  If the customer would still wish to test them, please let me know and I will be happy to set up testing discount codes. 

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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