重组Anti-Dishevelled 2 (phospho S143)抗体[EPNCIR145] (ab124933)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Dishevelled 2 (phospho S143)抗体[EPNCIR145]
参阅全部 Dishevelled 2 一抗 -
描述
兔单克隆抗体[EPNCIR145] to Dishevelled 2 (phospho S143) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab173138) -
阳性对照
- WB: F9 and HEK293 cell lysates.
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常规说明
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR145 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138).
For unpurified use at 1/1000 - 1/10000. |
说明 |
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WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138). For unpurified use at 1/1000 - 1/10000. |
靶标
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功能
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
序列相似性
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
结构域
The DIX domain mediates homooligomerization. -
细胞定位
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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数据库链接
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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别名
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
图片
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All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)
Lane 1 : Untreated F9 cell lysate
Lane 2 : Alkaline phosphatase treated F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)
Lane 1 : Untreated HEK293 cell lysate
Lane 2 : Alkaline phosphatase treated HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)
Lane 1 : F9 cell lysate untreated
Lane 2 : F9 cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab124933 被引用在 11 文献中.
- Bellchambers HM & Ware SM Loss of Zic3 impairs planar cell polarity leading to abnormal left-right signaling, heart defects and neural tube defects. Hum Mol Genet 30:2402-2415 (2021). PubMed: 34274973
- Mun H et al. The Autophagy Regulator p62 Controls PTEN-Dependent Ciliogenesis. Front Cell Dev Biol 8:465 (2020). PubMed: 32587859
- Peng Y & Wang HH Cir-ITCH inhibits gastric cancer migration, invasion and proliferation by regulating the Wnt/ß-catenin pathway. Sci Rep 10:17443 (2020). PubMed: 33060778
- Endo M et al. E2F1-Ror2 signaling mediates coordinated transcriptional regulation to promote G1/S phase transition in bFGF-stimulated NIH/3T3 fibroblasts. FASEB J 34:3413-3428 (2020). PubMed: 31922321
- Mei J et al. Nerve modulation therapy in gouty arthritis: targeting increased sFRP2 expression in dorsal root ganglion regulates macrophage polarization and alleviates endothelial damage. Theranostics 9:3707-3722 (2019). PubMed: 31281508