重组Anti-Diphtheria Toxin抗体[IGX3492R-3] (ab219834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IGX3492R-3] to Diphtheria Toxin
- Suitable for: WB
概述
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产品名称
Anti-Diphtheria Toxin抗体[IGX3492R-3]
参阅全部 Diphtheria Toxin 一抗 -
描述
兔单克隆抗体[IGX3492R-3] to Diphtheria Toxin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Corynebacterium diphtheriae -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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阳性对照
- WB: Diphtheria Toxin (mutated G52 E) full length protein
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常规说明
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
IGX3492R-3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219834于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
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说明 |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa). |
靶标
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相关性
Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. -
细胞定位
Secreted -
别名
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
图片
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Anti-Diphtheria Toxin antibody [IGX3492R-3] (ab219834) at 0.5 µg/ml +
Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg
Secondary
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab219834 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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