产品名称Anti-Desmin抗体[DE-U-10] - Cytoskeleton Marker
参阅全部 Desmin 一抗
描述小鼠单克隆抗体[DE-U-10] to Desmin - Cytoskeleton Marker
经测试应用适用于: WB, IHC-FoFr, IHC-P, IHC-Frmore details
不适用于: ICC or ICC/IF
预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
Full length native protein (purified) (Pig).
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Skeletal muscle.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab6322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Monoclonal anti-desmin may be used for Immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
功能Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
疾病相关Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
序列相似性Belongs to the intermediate filament family.
- Information by UniProt
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
ab6322 at 1/100 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incuabion with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.
Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322) at 1/3000 dilution + Mouse skeletal muscle tissue lysate at 30 µg
HRP-conjugated Goat anti Mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked with 5% Milk for 1 hour at 25°C.
Immunohistochemical analysis of Human colorectal tissue, staining Desmin (green) with ab6322.
Tissue was fixed with ice cold acetone for 5 minutes and blocked with 10% goat serum for 30 minutes. Samples were incubated with primary antibody (1/30) overnight at 4°C and an AlexaFluor®488®-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Gertz K et al. Partial loss of VE-cadherin improves long-term outcome and cerebral blood flow after transient brain ischemia in mice. BMC Neurol 16:144 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27538712) »
- Park SY et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun 7:10871 (2016). Read more (PubMed: 26972991) »