概述

  • 产品名称
    Anti-Dengue Virus NS1 glycoprotein抗体[DN1]
    参阅全部 Dengue Virus NS1 glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[DN1] to Dengue Virus NS1 glycoprotein
  • 宿主
    Mouse
  • 特异性
    Recognises the non-structural protein NS1 from Dengue Virus 2 (16681 strain). Reacts only weakly with NS1 from Dengue Virus 2 NGC strain, use ab41623 for detection of Dengue Virus 2 NGC NS1.
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Dengue virus 2
  • 免疫原

    Full length native protein purified from Dengue Virus 2 infected supernatant

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.065% Sodium azide
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    DN1
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP Use at an assay dependent dilution.
ICC/IF 1/5 - 1/20.

靶标

  • 相关性
    NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • 数据库链接
    • 别名
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    图片

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN1] (ab41490)

      Lane 1 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
      Lane 2 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
      Lane 3 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)
      Lane 4 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)

      Predicted band size: 40 kDa
      Observed band size: 40,80 kDa
      why is the actual band size different from the predicted?



      NS1 is present as a dimer (~80 kDa) in unheated samples but dissociates into monomers (~40 kDa) if samples are boiled before loading on the gel. Ab41490 recognises both forms of NS1.

    文献

    This product has been referenced in:
    • Callaway JB  et al. Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1ß Secretion: A Primary Human Monocyte Model. PLoS One 10:e0136708 (2015). Read more (PubMed: 26301593) »
    See 1 Publication for this product

    客户评价及客户问答

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in ELISA yet.

    Therefore, I can offer a discount off a future purchase if your customerbuysone of them now, tests it in ELISA and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value ofthe antibodyand can be used for the next order of any product.

    If your customer is interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and confirm which one of these antibodiesyour customer would like to testin ELISA . I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

    2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if teh antibody turns out to be unsuitable for ELISA, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glycoprotein antibody [DN3]):

    https://www.abcam.com/index.html?datasheet=41616 (or use the following: https://www.abcam.com/index.html?datasheet=41616).

    Heaton NS et al. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107:17345-50 (2010). ICC/IF. PubMed: 20855599

    Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
    https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

    ab41623 (Anti-Dengue Virus NS1 glycoprotein antibody [DN2]):

    https://www.abcam.com/index.html?datasheet=41623 (or use the following: https://www.abcam.com/index.html?datasheet=41623).

    Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF. PubMed: 21658241

    Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
    https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

    ab41490 (Anti-Dengue Virus NS1 glycoprotein antibody [DN1]):

    https://www.abcam.com/index.html?datasheet=41490 (or use the following: https://www.abcam.com/index.html?datasheet=41490).

    Currently we do not know of any references using this antibody.

    Isotype control: ab18449 (Mouse IgG2a, kappa [MOPC-173] (low endotoxin) - isotype control)
    https://www.abcam.com/index.html?datasheet=18449 (or use the following: https://www.abcam.com/index.html?datasheet=18449).

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

    For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    We do not sell directly to Pakistan. Please contact our trusted distributor for pricing information:

    Friends Scientific Corporation
    3-B Ali Continental,
    1-Mozang Road
    Lahore
    54000
    Pakistan

    Tel 4237236176 Fax 4237321500 Email mailto:friends1@wol.net.pk

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

    Answer

    Thank you for your reply. These three antibodies are provided as tissue culture supernatant. This is stated in the purity section of the datasheet. It has some sodium azide added to it, but is not provided in a storage buffer. I am sorry we do not know the concentration of these antibodies. Antibody concentration is usually determined by a protein assay. Because the tissue culture supernatant contains many other proteins, this would not be an accurate method in this case. We can estimate that tissue culture supernatantusually containsapproximately 1 - 3 mg/ml or antibody. I am sorry we are unable to provide more precise information on this occasion. However, I hope it is helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Answer

    Thank you for your enquiry. Wecan only confirm that ab41490 DN1 antibody will recognize NS1 protein from an MVA NS1 recombinant.I am sorry, we have no data from NS1 expressed in E coli or P pastoris However, if your recombinant proteins (ie NS1 from DENV2 strains 16681 or NGC) are folded properly,these antibodies would most likely work. Hence, these antibodies could be used to monitor proper folding of recombinant proteins. However, I am sorry we would not be able to guarantee this, and you would need to test this out yourself. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Answer

    Thank you for contacting Abcam. The culture supernatants are from hybridoma cultures and since it is not purified we do not have an estimate of the concentration of the mab in the supernatant which also contains other proteins. I am sorry that I was not able to provide you with the information that you required but if there is anything else I can help you with, please let me know.    

    Read More

    Answer

    For ab41490, you get a total of 1ml of solution per order. If there is anything else I can help you with, please let me know.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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