产品名称Anti-Dengue Virus NS1 glycoprotein抗体[DN1]
参阅全部 Dengue Virus NS1 glycoprotein 一抗
描述小鼠单克隆抗体[DN1] to Dengue Virus NS1 glycoprotein
特异性Recognises the non-structural protein NS1 from Dengue Virus 2 (16681 strain). Reacts only weakly with NS1 from Dengue Virus 2 NGC strain, use ab41623 for detection of Dengue Virus 2 NGC NS1.
经测试应用适用于: WB, IP, ICC/IFmore details
种属反应性与反应: Dengue virus 2
Full length native protein purified from Dengue Virus 2 infected supernatant
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.065% Sodium azide
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab41490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|IP||Use at an assay dependent dilution.|
|ICC/IF||1/5 - 1/20.|
相关性NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
- SwissProt: P29990 Dengue virus 2
- Dengue NS1 antibody
- Dengue Virus non structural protein 1 antibody
All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN1] (ab41490)
Lane 1 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
Lane 2 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
Lane 3 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)
Lane 4 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)
Predicted band size: 40 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
NS1 is present as a dimer (~80 kDa) in unheated samples but dissociates into monomers (~40 kDa) if samples are boiled before loading on the gel. Ab41490 recognises both forms of NS1.
This product has been referenced in:
- Callaway JB et al. Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1ß Secretion: A Primary Human Monocyte Model. PLoS One 10:e0136708 (2015). Read more (PubMed: 26301593) »