Anti-DIS3抗体(ab68570)
Key features and details
- Mouse polyclonal to DIS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DIS3抗体
参阅全部 DIS3 一抗 -
描述
小鼠多克隆抗体to DIS3 -
宿主
Mouse -
特异性
This antibody is reactive against mammalian transfected lysate. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human DIS3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa). |
靶标
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功能
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
细胞定位
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
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数据库链接
- Entrez Gene: 22894 Human
- Omim: 607533 Human
- SwissProt: Q9Y2L1 Human
- Unigene: 728935 Human
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别名
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
图片
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All lanes : Anti-DIS3 antibody (ab68570) at 1/500 dilution
Lane 1 : DIS3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 109 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab68570 被引用在 3 文献中.
- Mardakheh FK et al. Proteomics profiling of interactome dynamics by colocalisation analysis (COLA). Mol Biosyst 13:92-105 (2016). PubMed: 27824369
- Pfeiffer JR et al. CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. Mol Cell Biol 31:277-86 (2011). ICC/IF ; Mouse . PubMed: 21078874
- Ayala YM et al. TDP-43 regulates its mRNA levels through a negative feedback loop. EMBO J 30:277-88 (2011). WB ; Human . PubMed: 21131904