Anti-DGKA抗体(ab88672)
Key features and details
- Mouse polyclonal to DGKA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DGKA抗体
参阅全部 DGKA 一抗 -
描述
小鼠多克隆抗体to DGKA -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human DGKA. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human spleen lysate; Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. -
组织特异性
Lymphocytes and oligodendroglial cells. -
序列相似性
Belongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers. - Information by UniProt
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数据库链接
- Entrez Gene: 1606 Human
- Omim: 125855 Human
- SwissProt: P23743 Human
- Unigene: 524488 Human
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别名
- 80 kDa diacylglycerol kinase antibody
- DAG kinase alpha antibody
- DAGK antibody
see all
图片
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Anti-DGKA antibody (ab88672) at 1 µg/ml + Human spleen lysate at 50 µg
Predicted band size: 83 kDa -
All lanes : Anti-DGKA antibody (ab88672) at 1 µg/ml
Lane 1 : DGKA transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 83 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted? -
Immunofluorescence staining of DGKA in HeLa cells using ab88672 at 10 µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab88672 被引用在 1 文献中.
- Lupino E et al. I?B kinase ß is required for activation of NF-?B and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells. J Immunol 188:2545-55 (2012). Human . PubMed: 22331067