存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab59493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml.|
功能Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
组织特异性Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
通路Glycerolipid metabolism; triacylglycerol biosynthesis.
序列相似性Belongs to the diacylglycerol acyltransferase family.
细胞定位Endoplasmic reticulum membrane.
- Information by UniProt
- DGAT2 antibody
- DGAT2_HUMAN antibody
- Diacylglycerol O acyltransferase like protein 2 antibody
ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml
Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
Lane 3 : Tissue lysates prepared from human liver tissue.
Observed band size: 45,60 kDa why is the actual band size different from the predicted?
Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg
Primary incubation was 1 hour.