Anti-DDX18抗体(ab70526)
Key features and details
- Rabbit polyclonal to DDX18
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX18抗体
参阅全部 DDX18 一抗 -
描述
兔多克隆抗体to DDX18 -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
A region between residues 1 and 50 of human DDX18 (NP_006764.3)
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阳性对照
- Whole cell lysate from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70526于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
IP |
说明 |
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WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Probable RNA-dependent helicase. -
序列相似性
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8886 Human
- Omim: 606355 Human
- SwissProt: Q9NVP1 Human
- Unigene: 728867 Human
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别名
- ATP dependent RNA helicase DDX18 antibody
- ATP-dependent RNA helicase DDX18 antibody
- DDX 18 antibody
see all
图片
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All lanes : Anti-DDX18 antibody (ab70526) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes -
Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70526 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab70526 尚未被引用在任何文献中。