PE Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2] (ab72469)
Key features and details
- PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: Flow Cyt, Sandwich ELISA
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
PE Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
PE小鼠单克隆抗体[M2] to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt, Sandwich ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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常规说明
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
Size exclusion chromatography as well as proprietary methods for purifying the final materials were used. -
克隆
单克隆 -
克隆编号
M2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
- Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt
Use at an assay dependent concentration. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Sandwich ELISA
Use at an assay dependent concentration. |
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
图片
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Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.
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Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab72469 被引用在 8 文献中.
- Meyer M et al. Structure-function relationships of the disease-linked A218T oxytocin receptor variant. Mol Psychiatry 27:907-917 (2022). PubMed: 34980886
- Edelstein HI et al. Elucidation and refinement of synthetic receptor mechanisms. Synth Biol (Oxf) 5:ysaa017 (2020). PubMed: 33392392
- Sharma S et al. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res 28:1372-1382 (2018). PubMed: 29914970
- AlHajri SM et al. Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion. J Virol 91:N/A (2017). Flow Cyt . PubMed: 28053110
- Leshchiner ES et al. Small-molecule inhibitors directly target CARD9 and mimic its protective variant in inflammatory bowel disease. Proc Natl Acad Sci U S A 114:11392-11397 (2017). PubMed: 29073062
- Compton AA et al. Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity. EMBO Rep 17:1657-1671 (2016). PubMed: 27601221
- Raphel L et al. Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells. PLoS One 7:e44052 (2012). Flow Cyt . PubMed: 22937150
- Maloveste SM et al. Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells. J Immunol 189:4338-48 (2012). PubMed: 23041569