Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
2 µg
Specification
chimeric protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS tween 0.05%
Dilution
1/1500
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 41 kDa
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提交于 Nov 17 2010