Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (olfactory epithelium)
Specification
olfactory epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.03% triton x100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Dilution
1/200
Additional data
Additional Notes
Nuclei are counterstained with DAPI (blue)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 18 2011