Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected staining pattern under these conditions - rather it produces a strong nuclear-enriched staining pattern. MeOH-fixed samples were also evaluate and did not produce the expected staining pattern.
Abcam response
Thank you for submitting this review. We have followed up with the customer since this should have worked in human cells IF staining. For more details about our Abpromise guarantee please check this link: www.abcam.com/abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Apr 04 2014