概述

  • 产品名称
  • 描述
    山羊多克隆抗体to CrkL
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, ICC, ELISA, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    KIFDPQNPDENE

    , corresponding to C terminal amino acids 292-303 of Human CrkL

  • 阳性对照
    • Recombinant Human CrkL protein (ab116208) can be used as a positive control in WB.

性能

应用

Our Abpromise guarantee covers the use of ab1302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 33 kDa.
ICC Use a concentration of 2.5 - 3.8 µg/ml.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent concentration.

靶标

图片

  • Anti-CrkL antibody (ab1302) at 0.01 µg/ml + K562 lysate (35µg protein in RIPA buffer).

    Predicted band size: 33 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab1302 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

文献

ab1302 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab1302.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册