重组Anti-EPF抗体[EPR4475] (ab108611)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4475] to EPF
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EPF抗体[EPR4475]
参阅全部 EPF 一抗 -
描述
兔单克隆抗体[EPR4475] to EPF -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4475 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108611于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. -
序列相似性
Belongs to the GroES chaperonin family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3336 Human
- Entrez Gene: 25462 Rat
- Omim: 600141 Human
- SwissProt: P61604 Human
- SwissProt: P26772 Rat
- Unigene: 1197 Human
- Unigene: 106093 Rat
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别名
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
see all
图片
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All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108611 被引用在 1 文献中.
- Kovács ÁF et al. Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation. Int J Mol Sci 20:N/A (2019). PubMed: 31337116