Choline/Acetylcholine Assay试剂盒(ab65345)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture extracts, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 nmol/well
概述
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产品名称
Choline/Acetylcholine Assay试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.01 nmol/well -
范围
0.01 nmol/well - 5 nmol/well -
检测时间
0h 40m -
种属反应性
与反应: Mammals, Other species -
产品概述
Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.
In the choline / acetylcholine assay protocol, free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm).
Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction to measure total choline (choline + acetyl choline). The amount of acetyl choline is calculated by subtracting choline from total choline.
There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.
Cells should be cultured in choline chloride free medium.
Choline / acetylcholine assay protocol summary:
- add samples and standards to wells
- add choline reaction mix with, or without, acetylcholinesterase, and incubate for 30 min at room temp
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K615 Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit. K615-100 is the same size as the 100 test size of ab65345.
If you need to purchase additional Choline Probe for this product, please see ab139505.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests Acetylcholinesterase Enzyme 1 vial 20 vials Assay Buffer VI 1 x 25ml 20 x 25ml Choline Standard 1 vial 20 vials Enzyme Mix IV 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl -
研究领域
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相关性
Choline and acetylcholine (often abbreviated ACh) play important roles in many biological processes. Choline is a natural amine, and is generally found in phosphatidylcholine and sphingomyelin, phopholipids abundant in cell membranes. Choline is also the precursor molecule for the neurotransmitter acetylcholine which is involved in many functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS). In the PNS, ACh activates muscles and is a major neurotransmitter in the autonomic nervous system. In the CNS, ACh and the associated neurons form the cholinergic system, which tends to cause anti-excitatory actions.
图片
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Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).
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Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells
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Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab65345 被引用在 56 文献中.
- Fielding C et al. Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis. Nat Commun 13:543 (2022). PubMed: 35087060
- Zhang H et al. The Effect of Guilingji Capsules on Vascular Mild Cognitive Impairment: A Randomized, Double-Blind, Controlled Trial. Evid Based Complement Alternat Med 2022:4778163 (2022). PubMed: 35116067
- Gadomski S et al. A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise. Cell Stem Cell 29:528-544.e9 (2022). PubMed: 35276096
- Moyat M et al. Microbial regulation of intestinal motility provides resistance against helminth infection. Mucosal Immunol 15:1283-1295 (2022). PubMed: 35288644
- Li Q et al. Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance. Cell Rep 40:111308 (2022). PubMed: 36044853