存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
ab2845 at a 1:10000 dilution staining ~57 kDa Chk 1 in HeLa whole cell lysate (30
µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds exposure). ab2845 at a 1:10000 dilution staining ~57 kDa Chk 1 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds exposure).
All lanes : Anti-Chk1 antibody (ab2845) at 1/10000 dilution
Lane 1 : Whole cell lysate of HT29 tumour cell line, untreated
Lane 2 : Whole cell lysate of HT29 tumour cell line, treated with DMSO
Lane 3 : Whole cell lysate of HT29 tumour cell line, treated with 50nM SN38 for 6h
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated cow anti-goat antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Nawrocki ST et al. The NEDD8-activating enzyme inhibitor MLN4924 disrupts nucleotide metabolism and augments the efficacy of cytarabine. Clin Cancer Res 21:439-47 (2015). Human . Read more (PubMed: 25388161) »
- Reeder A et al. Stress hormones reduce the efficacy of paclitaxel in triple negative breast cancer through induction of DNA damage. Br J Cancer 112:1461-70 (2015). WB . Read more (PubMed: 25880007) »