参阅全部 Chk1 一抗
描述兔单克隆抗体[E250] to Chk1
特异性ab32531 recognises Chk1.
经测试应用适用于: ICC/IF, WB, Flow Cyt, IPmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide within Human Chk1 aa 1-100 (N terminal). The exact sequence is proprietary.
- K562 cell lysate
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
Anti-Chk1 antibody [E250] (ab32531) at 1/1000 dilution + K562 cell lysate
Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Chk1 with Purified ab32531 at 1/250 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling Chk1 with unpurified ab32531 at 1/200 dilution (1µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This product has been referenced in:
- Liu H et al. The Deubiquitylating Enzyme USP4 Cooperates with CtIP in DNA Double-Strand Break End Resection. Cell Rep 13:93-107 (2015). Read more (PubMed: 26387952) »
- Luo MH et al. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol 81:1934-50 (2007). WB ; Human . Read more (PubMed: 17151099) »