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ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet.
Species raised in
|Isotype||Protein A binding affinity||Protein G binding affinity|
|IgM||Use anti-mouse IgM|
|Buffer A||1 x 10ml|
|Buffer B||1 x 30ml|
|1.25M Glycine||1 x 10ml|
|Buffer C||1 x 30ml|
|Buffer D||1 x 3ml|
|ChIP Buffer 5X||1 x 84ml|
|DNA Purifying Slurry||1 x 3ml|
|ab1791 - H3 antibody||1 x 25µg|
|PCR-Grade Water||1 x 10.2ml|
|Protease Inhibitors||1 tablet|
|Protein A Beads Unblocked||1 x 960µl|
|Proteinase K||1 x 30µl|
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"