Cathepsin D抑制剂Assay 试剂盒(ab126779)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Inhibitor compounds
概述
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产品名称
Cathepsin D抑制剂Assay 试剂盒
参阅全部 Cathepsin D 试剂盒 -
检测方法
Fluorescent -
样品类型
Inhibitor compounds -
检测类型
Quantitative -
检测时间
1h 00m -
产品概述
Cathepsin D Inhibitor Screening Kit (ab126779) is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group MCA, which can then easily be measured using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K148 Cathepsin D Inhibitor Screening Kit (Fluorometric). K148-100 is the same size as the 100 test size of ab126779.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate pro-apoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 100 tests CD Reaction Buffer 1 x 10ml Human Cathepsin D 1 vial Pepstatin A Solution 1 x 20µl Substrate II 1 x 0.2ml -
研究领域
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相关性
Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N linked oligosaccharides. Cathepsin D is synthesized as an inactive pro enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer and possibly Alzheimer's disease. -
细胞定位
Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. -
别名
- CLN10
- CPSD
- CTSD
- Lysosomal aspartyl protease
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126779 被引用在 1 文献中.
- Qiao S et al. The antimalarial amodiaquine causes autophagic-lysosomal and proliferative blockade sensitizing human melanoma cells to starvation- and chemotherapy-induced cell death. Autophagy 9:2087-102 (2013). PubMed: 24113242