Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: skimmed milk 5%
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL
Bands
Specific: 35 kDa
Exposure
10 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 04 2013