概述

  • 产品名称
    Anti-Caspase-3抗体
    参阅全部 Caspase-3 一抗
  • 描述
    兔多克隆抗体to Caspase-3
  • 宿主
    Rabbit
  • 特异性
    Caspase-3 is a member of the interleukin-1 ß-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating t-o subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.
  • 经测试应用
    适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-3 aa 167-175. Corresponding to the cleavage site of human caspase 3.
    Database link: P42574

  • 阳性对照
    • Tonsil

性能

应用

Our Abpromise guarantee covers the use of ab4051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa. PubMed: 24367539
IHC-P Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-Fr Use at an assay dependent concentration. PubMed: 24465746

靶标

  • 功能
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 组织特异性
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性
    Belongs to the peptidase C14A family.
  • 翻译后修饰
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

图片

  • ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

  • Anti-Caspase-3 antibody (ab4051) + Human Lung Cell Extract

    Predicted band size: 32 kDa

  • ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Ali BH  et al. Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon. Am J Transl Res 10:126-137 (2018). Read more (PubMed: 29422999) »
  • Liao D  et al. Dysregulation of Neuregulin-1/ErbB signaling in the hippocampus of rats after administration of doxorubicin. Drug Des Devel Ther 12:231-239 (2018). Read more (PubMed: 29430172) »
See all 90 Publications for this product

客户评价及客户问答

1-10 of 23 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA High pH
Permeabilization
No
Specification
Tonsil
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Intestines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Intestines
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Sep 20 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (intestine)
Antigen retrieval step
None
Permeabilization
No
Specification
intestine
Fixative
10% NBF

Abcam user community

Verified customer

提交于 Jul 05 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Gingiva)
Permeabilization
Yes - Triton X-100
Specification
Gingiva
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

提交于 Apr 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Cow Tissue sections (Ovary)
Specification
Ovary
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 24 2014

Answer





1. can it be shipped at room ? ab137994 is shipped on wet ice at 4 degrees C.


2. How long will it be valid after its opening? I am asking because i want to test 20 samples then after 8months test rest of samples..can i do that or the kit will be expired? ==== It will probably be OK to store this kit for 8 months between tests. Please read the Protocol Booklet sections 9 and 10 on Assay Preparation and Reagent Preparation as specific storage requirements of prepared reagents are described. For example, only diluite enough 'Diluent N' required because once diluted it can only be stored for 1 month at 4 degrees C. Also, only use 1 vial of Standard with the first round of samples, so you can store the second vial for use in 8 months with the next round of samples. This is because once diluted, the Standard can only be stored for 30 days at -20 degrees C.


3. What is the size of this kit? ==== This kit provides reagents sufficient for 96 tests, which is not the same thing as 96 samples. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. Review the protocol completely to confirm this kit meets your requirements.

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Answer

Thank you for your enquiry and your interest in our products.

As the datasheet indicates ab4051 has been tested for both IHC-P (paraffin embedded formallin fixed tissues) and IHC-Fr (frozen tissues).

If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting Abcam.

The concentration of ab4051 lot XXXXXX is XXX mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question
Answer

Thank you for contacting us. I have been in contact with teh lab regarding concnetrations of the antibodies which we spoke of earlier today. AB4051, rabbit polyclonal to Caspase 3, lot xxx has a concentration of xxxxmg/mL AB32152, rabbit monoclonal to VEGF Receptor 1, lot xxxx has a concentration of xxxmg/mL. Please keep in mind that rabbit monoclonals have a much higher affinity (10x-100x) than monoclonal antibodies from other species. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

ab4051 lot GR78964-1 has a concentration of: 0.31 mg/ml.

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1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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