Application
Western blot
Sample
Human Cell lysate - whole cell (LX-2 Human Hepatic Stellate Cell Line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
LX-2 Human Hepatic Stellate Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 40, 42, 33, 31 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 14 2017