Application
Western blot
Sample
Pig Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: rt°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit 680 LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
Fluorescence NIR
Exposure
2 second(s)
Bands
Specific: 37 KDa kDa
Additional data
Additional Notes
Positive detection of swine TNNT2 with similar specificity to ist human ortholog
Figure legend: TNNT2 expression in left ventricle total protein lysates of two pig (lanes 1 and 2) and one human samples (lanes 3, 4) detected with decreasing concentrations of ab91605.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 25 2016