重组Anti-Cardiac Troponin T抗体[EPR3695] (ab91605)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3695] to Cardiac Troponin T
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cardiac Troponin T抗体[EPR3695]
参阅全部 Cardiac Troponin T 一抗 -
描述
兔单克隆抗体[EPR3695] to Cardiac Troponin T -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cardiac Troponin T aa 50-150. The exact sequence is proprietary.
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阳性对照
- IP: Human heart lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3695 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91605于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Predicted molecular weight: 36 kDa.
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IP |
1/50.
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only. |
说明 |
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WB
1/1000. Predicted molecular weight: 36 kDa. |
IP
1/50. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only. |
靶标
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功能
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
组织特异性
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
疾病相关
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
序列相似性
Belongs to the troponin T family. - Information by UniProt
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数据库链接
- Entrez Gene: 7139 Human
- Omim: 191045 Human
- SwissProt: P45379 Human
- Unigene: 533613 Human
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别名
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labelling Cardiac Troponin T with purified ab91605 at 1/500 dilution (0.32 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/1000 dilution (Purified)
Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15793653).
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Purified ab91605 at 1/50 dilution (2µg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab91605 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab91605 被引用在 13 文献中.
- Fagg WS et al. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic Acids Res 50:5313-5334 (2022). PubMed: 35544276
- Tang Y et al. Inhibition of EZH2 primes the cardiac gene activation via removal of epigenetic repression during human direct cardiac reprogramming. Stem Cell Res 53:102365 (2021). PubMed: 34087994
- Chen W et al. TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction. JCI Insight 6:N/A (2021). PubMed: 34676829
- Wu X et al. Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction. Front Cardiovasc Med 8:768873 (2021). PubMed: 34805322
- Jing L et al. TNNT2 as a potential biomarker for the progression and prognosis of colorectal cancer. Oncol Rep 44:628-636 (2020). PubMed: 32627044