Calpain activity kit tested in rat's gastrocnemius (fast fibers). 2 conditions: control and 7 days of sepsis.
10mg of muscle was crushed with ultraturax on ice in the extraction buffer of the kit. After a centrifugation (4min, 4°C, 11000rpm) proteins were quantify in supernatant by a Bradford test. The kit was made according to the instructions, samples in duplicate and read at 405/510nm.
the figure explain the % of variation of calpain activity in septic muscle compare to control group.
No difference was observed
10mg of muscle was crushed with ultraturax on ice in the extraction buffer of the kit. After a centrifugation (4min, 4°C, 11000rpm) proteins were quantify in supernatant by a Bradford test. The kit was made according to the instructions, samples in duplicate and read at 405/510nm.
the figure explain the % of variation of calpain activity in septic muscle compare to control group.
No difference was observed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Florine Tissier
Verified customer
提交于 Feb 12 2018