Calcium Assay试剂盒(Colorimetric) (ab102505)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mM
概述
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产品名称
Calcium Assay试剂盒(Colorimetric)
参阅全部 Calcium 试剂盒 -
样品类型
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
检测类型
Quantitative -
灵敏度
> 0.1 mM -
范围
0.1 mM - 25 mM -
检测时间
0h 20m -
种属反应性
与反应: Mammals, Other species -
产品概述
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM).
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
This calcium assay detects free calcium ions only.
Calcium assay protocol summary:
- add samples and standards to wells
- add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
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平台
Microplate reader
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 250 tests Calcium Assay Buffer 1 x 15ml Calcium Standard 1 x 100µl Chromogenic Reagent 1 x 25ml -
研究领域
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相关性
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
别名
- Ca++
- Ca2+
相关产品
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Corresponding kit
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Related Products
图片
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Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
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Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
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Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
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Example of standard curve obtained using ab102505
数据表及文件
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SDS download
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Datasheet download
文献 (106)
ab102505 被引用在 106 文献中.
- Phan K et al. Multiple pathways of lipid dysregulation in amyotrophic lateral sclerosis. Brain Commun 5:fcac340 (2023). PubMed: 36632187
- Yadav MK et al. Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice. Mol Ther Methods Clin Dev 27:149-166 (2022). PubMed: 36284768
- Martino E et al. Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death. Nutrients 14:N/A (2022). PubMed: 36501111
- Chao CT et al. Deep Learning-Assisted Repurposing of Plant Compounds for Treating Vascular Calcification: An In Silico Study with Experimental Validation. Oxid Med Cell Longev 2022:4378413 (2022). PubMed: 35035662
- Shi LL et al. Liraglutide Alleviates Diabetic Atherosclerosis through Regulating Calcification of Vascular Smooth Muscle Cells. Dis Markers 2022:5013622 (2022). PubMed: 35510038