Anti-COX2 / Cyclooxygenase 2抗体(ab15191)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, Sandwich ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-COX2 / Cyclooxygenase 2抗体
参阅全部 COX2 / Cyclooxygenase 2 一抗 -
描述
兔多克隆抗体to COX2 / Cyclooxygenase 2 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, Sandwich ELISA, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Syrian hamster -
免疫原
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35355 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (19) |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
|
Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
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IHC-P | (8) |
1/100.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
IHC-P
1/100. |
靶标
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功能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻译后修饰
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
别名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
图片
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All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml
Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Exposure time: 10 seconds -
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
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ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (394)
ab15191 被引用在 394 文献中.
- Lee H et al. Protective Effect of Gamma Aminobutyric Acid against Aggravation of Renal Injury Caused by High Salt Intake in Cisplatin-Induced Nephrotoxicity. Int J Mol Sci 23:N/A (2022). PubMed: 35008928
- Liu TT et al. Efficacy profile of noninvasive vagus nerve stimulation on cortical spreading depression susceptibility and the tissue response in a rat model. J Headache Pain 23:12 (2022). PubMed: 35062860
- Qiao W et al. Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models. Nat Commun 13:535 (2022). PubMed: 35087048
- De Matteis R et al. Aspirin activates resolution pathways to reprogram T cell and macrophage responses in colitis-associated colorectal cancer. Sci Adv 8:eabl5420 (2022). PubMed: 35108049
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741