Application
Western blot
Sample
Rat Tissue lysate - whole (kidney)
Loading amount
20 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (10-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST(0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: A9044
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 40 second(s)
Bands
Specific: 90, 75, 50 kDa Non-specific: 25 kDa
Additional data
Additional Notes
Lanes in the picture: 1 and 2, rat kidney outer medulla homogenate samples, 3 and 4, dual color protein standard from biorad (161-0374).
protein standard molecular weight: 250, 150,100,75(quite weak) 50,37,25(unvisible),20,15(weak).
protein standard molecular weight: 250, 150,100,75(quite weak) 50,37,25(unvisible),20,15(weak).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 11 2013