产品名称Anti-CKII alpha (phospho T360 + S362)抗体
参阅全部 CKII alpha 一抗
描述兔多克隆抗体to CKII alpha (phospho T360 + S362)
特异性ab119410 detects endogenous levels of Human CKII alpha only when phosphorylated at Threm=onine 360/Srine 362
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide conjugated to KLH, surrounding amino acids (VTPpPSpPL) phosphorylated at threonine 360 and Serine 362 of Human CKII alpha (NP_001894.2).
- Recombinant proteins CKII alpha wt, CKII alpha T360A, CKII alpha S362A, CKII alphaT360/S362A phoshorylated by ERK2
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: (without Mg2+ and Ca2+)
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab119410 was purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab119410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).|
功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase II alpha 1 antibody
- Casein kinase II alpha 1 subunit antibody
All lanes : Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution
Lane 1 : CKII alpha
Lane 2 : CKII alpha + ERK2
Lane 3 : CKII alpha T360A
Lane 4 : CKII alpha T360A + ERK2
Lane 5 : CKII alpha S362A
Lane 6 : CKII alpha S362A + ERK2
Lane 7 : CKII alpha T360/S362A
Lane 8 : CKII alpha T360/S362A + ERK2
Predicted band size: 45 kDa
Lower panel stained with CKII alpha antibody as a loading control.
Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution + C57BL/6 Mouse splenocyte whole cell lysate at 40 µg
HRP-conjugated goat anti-rabbit monoclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 minute
Blocked with 5% milk for 1 hour at 25°C
ab119410 has not yet been referenced specifically in any publications.