Application
Western blot
Sample
Rat Tissue lysate - whole (heart (left ventricle))
Gel Running Conditions
Reduced Denaturing (15% poly-acrylamide)
Loading amount
20 µg
Specification
heart (left ventricle)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1:1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: ECL Anti-mouse IgG, peroxidase-linked whole antibo
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: greater than 70 kDa
Positive control
rat liver lysate
Additional data
Additional Notes
>70kD size bands appeared instead of the correct 16kD bands. No bands are shown at 16kD position.
The picture shows exposure after 1 hour primary antibody incubation at room temperature.
Abcam response
Thank you for your honest feedback. This antibody has only been tested using a recombinant protein expressed in E. coli. We have no data on the detection of the endogenous protein. We would welcome any feedback from other customers using this antibody in western blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 07 2012