Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver, spleen)
Loading amount
80 µg
Specification
Liver, spleen
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: N/A kDa Non-specific: 70 kDa
Positive control
3T3-L1 cell lysate
Negative control
N/A
Additional data
Additional Notes
The antibody did not work for mouse liver, spleen, heart and adipose.
Abcam response
Thank you for your honest feedback. This antibody has only been tested using a recombinant protein expressed in E. coli. We have no data on the detection of the endogenous protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 20 2009