Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spleen)
Specification
Spleen
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Anti-Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
10um frozen section. Sections fixed for 10mins in 4%PFA.
Wash 3x3min in PBS.
Block 10% donkey serum for 20min at RT.
Primary Ab diluted 1/100 in PBS and incubated overnight at 4deg, also works by incubating for 2 hours at RT.
Wash 3x3min in PBS.
Secondary - donkey anti-mouse AF488 (green) 1/1000 in PBS for 1h at RT.
Wash 3x3min in PBS
Counterstained with Hoechst 33258 (blue) for 10mins at RT.
Wash 3min in dH2O
Mounted in Invitrogen ProLong gold and kept at 4deg until imaged.
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提交于 Sep 06 2011