存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab118307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use a concentration of 5 µg/ml.|
功能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
组织特异性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
序列相似性Belongs to the tetraspanin (TM4SF) family.
细胞定位Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
- Information by UniProt
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
ICC/IF image of ab118307 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118307, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-CD63 antibody (ab118307) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg
Predicted band size: 26 kDa
Sample Type: mouse fibroblast lysate (20µg) Primary Dilution (ab118307): 1:800 (2% milk) Secondary Dilution: 1:2000 (5% milk)
This product has been referenced in:
- Peng Y et al. Human embryonic stem cells extracellular vesicles and their effects on immortalized human retinal Müller cells. PLoS One 13:e0194004 (2018). Read more (PubMed: 29538408) »
- Ghadially H et al. MHC class I chain-related protein A and B (MICA and MICB) are predominantly expressed intracellularly in tumour and normal tissue. Br J Cancer 116:1208-1217 (2017). Read more (PubMed: 28334733) »