Anti-CD62E抗体(ab18981)
Key features and details
- Rabbit polyclonal to CD62E
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD62E抗体
参阅全部 CD62E 一抗 -
描述
兔多克隆抗体to CD62E -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
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阳性对照
- WB: Jurkat cell lysate. Flow Cyt: Jurkat cells. IHC-P: Human placenta
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3631 E-Selectin Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.15% EDTA -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18981于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa. |
Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
序列相似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6401 Human
- Entrez Gene: 25544 Rat
- Omim: 131210 Human
- SwissProt: P16581 Human
- SwissProt: P98105 Rat
- Unigene: 82848 Human
- Unigene: 10359 Rat
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别名
- CD26E antibody
- CD62 antigen-like family member E antibody
- CD62E antibody
see all
图片
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Anti-CD62E antibody (ab18981) at 1/1000 dilution + Jurkat cell lysate at 80 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
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ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Flow cytometric analysis of Jurkat cells using ab18981 (red) with negative controls (black and green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab18981 被引用在 38 文献中.
- García-Bernal D et al. Enforced mesenchymal stem cell tissue colonization counteracts immunopathology. NPJ Regen Med 7:61 (2022). PubMed: 36261464
- Ying Y et al. Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAG. Aging (Albany NY) 13:8628-8642 (2021). PubMed: 33714955
- Li C et al. Chronic Low-Dose Alcohol Consumption Attenuates Post-Ischemic Inflammation via PPAR? in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34066125
- Williams H et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal 15:421-432 (2021). PubMed: 34080128
- Liu S et al. Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway. Evid Based Complement Alternat Med 2021:5881321 (2021). PubMed: 34912465